Hotspot

rps19-rpl22

IRa · 161743-161801 · 59 bp

Back to Macleaya

Pi 0.417

MarkerSeek score 65.50

Primer available yes

Label rps19-rpl22

Feature Metadata

label_name: rps19-rpl22
feature_type: igs
region: IRa
length_bp: 59
variable_sites: 10
pi: 0.417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.41
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl22_p1	TGGGTAGAGCCGGATCTAAG	CGCTTTCTCCATTAGCCCAT	55.0	50.0	58.0	58.0	1104 bp	0.5	59.8
rps19-rpl22_p2	CCACAATGATCGGCCATACA	CGCTTTCTCCATTAGCCCAT	50.0	50.0	58.0	58.0	698 bp	0.5	59.5
rps19-rpl22_p3	TGGGTAGAGCCGGATCTAAG	CGTTGACTTCGGCTTGACTA	55.0	50.0	58.0	57.9	873 bp	0.5	59.5
rps19-rpl22_p4	TGGGTAGAGCCGGATCTAAG	ATTCCCTCGACACCTGAAGA	55.0	50.0	58.0	58.1	1314 bp	0.5	59.5
rps19-rpl22_p5	TGGGTAGAGCCGGATCTAAG	TTCCCTCGACACCTGAAGAT	55.0	50.0	58.0	58.1	1313 bp	0.5	59.5