Hotspot

ccsA-ndhD

SSC · 121858-122118 · 261 bp

Back to Macleaya

Pi 0.0478

MarkerSeek score 75.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 261
variable_sites: 12
pi: 0.0478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGGGCTACATAGTTATGGTTCA	TCCGGATTTCGTTCTCTCAC	40.9	50.0	56.3	57.4	374-384 bp	1.0	50.0
ccsA-ndhD_p2	AGGGCTACATAGTTATGGTTCA	CCGGATTTCGTTCTCTCACT	40.9	50.0	56.3	57.3	373-383 bp	1.0	50.0
ccsA-ndhD_p3	AGGGCTACATAGTTATGGTTCAT	TCCGGATTTCGTTCTCTCAC	39.1	50.0	56.8	57.4	374-384 bp	1.0	46.1
ccsA-ndhD_p4	AGGGCTACATAGTTATGGTTCAT	CCGGATTTCGTTCTCTCACT	39.1	50.0	56.8	57.3	373-383 bp	1.0	46.0
ccsA-ndhD_p5	AGGGCTACATAGTTATGGTTCA	ATCCGGATTTCGTTCTCTCAC	40.9	47.6	56.3	57.8	375-385 bp	1.0	45.9