Hotspot

petA-psbJ

LSC · 67863-68983 · 1121 bp

Back to Macleaya

Pi 0.0179

MarkerSeek score 54.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1121
variable_sites: 20
pi: 0.0179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGACGCCAAGTGGTTGATAT	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1957-1959 bp	1.0	80.8
petA-psbJ_p2	GGACGCCAAGTGGTTGATAT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1956-1958 bp	1.0	80.8
petA-psbJ_p3	GGACGCCAAGTGGTTGATAT	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1743-1745 bp	1.0	80.6
petA-psbJ_p4	GGACGCCAAGTGGTTGATAT	GGACTAGCTGTACCTACCGT	50.0	55.0	58.0	58.0	1839-1841 bp	1.0	80.5
petA-psbJ_p5	GGACGCCAAGTGGTTGATAT	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.0	58.0	1981-1983 bp	1.0	80.4