Hotspot

trnT-GGU-psbD

LSC · 34467-36070 · 1604 bp

Back to Macleaya

Pi 0.0144

MarkerSeek score 62.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1604
variable_sites: 23
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AATTGAATCAAACGGGCCCT	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.1	58.2	1778-1783 bp	1.0	78.9
trnT-GGU-psbD_p2	ATTGAATCAAACGGGCCCTT	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.1	58.2	1777-1782 bp	1.0	78.9
trnT-GGU-psbD_p3	AATTGAATCAAACGGGCCCT	GACCGGACCAACCTACAAAA	45.0	50.0	58.1	57.7	1792-1797 bp	1.0	78.8
trnT-GGU-psbD_p4	ATTGAATCAAACGGGCCCTT	GACCGGACCAACCTACAAAA	45.0	50.0	58.1	57.7	1791-1796 bp	1.0	78.8
trnT-GGU-psbD_p5	AACGGGCCCTTTTAACTCAG	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1768-1773 bp	1.0	78.7