Hotspot

trnE-UUC-trnT-GGU

LSC · 33848-34394 · 547 bp

Back to Macleaya

Pi 0.0241

MarkerSeek score 66.00

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 12
pi: 0.0241
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	611-650 bp	1.0	83.4
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	590-629 bp	1.0	83.3
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.3	57.9	576-615 bp	1.0	82.7
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.7	57.9	555-594 bp	1.0	82.6
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	TGGAAAAAGCCCCTTATCGG	55.0	50.0	58.3	57.9	635-674 bp	1.0	82.3