Hotspot

trnD-GUC-trnY-GUA

LSC · 33237-33631 · 395 bp

Back to Macleaya

Pi 0.0211
MarkerSeek score 65.50
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
395
variable_sites
8
pi
0.0211
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 TTCATCGGATCCTAGGTCGG ATGGATTCTTCCTGGGTCGA 55.0 50.0 58.4 58.1 567-583 bp 1.0 79.3
trnD-GUC-trnY-GUA_p2 TTCATCGGATCCTAGGTCGG TGGATTCTTCCTGGGTCGAT 55.0 50.0 58.4 58.1 566-582 bp 1.0 79.3
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA ATGGATTCTTCCTGGGTCGA 50.0 50.0 58.5 58.1 506-522 bp 1.0 79.0
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA TGGATTCTTCCTGGGTCGAT 50.0 50.0 58.5 58.1 505-521 bp 1.0 79.0
trnD-GUC-trnY-GUA_p5 TTCATCGGATCCTAGGTCGG TGGCAATATGTCTACGCTGG 55.0 50.0 58.4 57.8 510-526 bp 1.0 78.7