Hotspot

petN-psbM

LSC · 30701-31884 · 1184 bp

Back to Macleaya

Pi 0.0104

MarkerSeek score 67.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1184
variable_sites: 11
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.0	57.6	1243-1339 bp	1.0	73.4
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	1249-1345 bp	1.0	71.9
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1189-1285 bp	1.0	70.6
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTC	45.0	45.0	58.0	56.1	1243-1339 bp	1.0	69.2
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTC	50.0	45.0	57.8	56.1	1249-1345 bp	1.0	67.7