Hotspot

trnS-GCU-trnG-UCC

LSC · 9633-10579 · 947 bp

Back to Macleaya

Pi 0.012

MarkerSeek score 67.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 947
variable_sites: 11
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	AACCGAAAGACCCCTTAACT	45.0	45.0	58.0	56.4	1041-1059 bp	1.0	69.9
trnS-GCU-trnG-UCC_p2	AGGAAACGGAAAGAGAGGGA	AACCGAAAGACCCCTTAACT	50.0	45.0	58.0	56.4	1095-1113 bp	1.0	69.8
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	AACCGAAAGACCCCTTAACT	50.0	45.0	58.2	56.4	1090-1108 bp	1.0	68.8
trnS-GCU-trnG-UCC_p4	GAGGAAACGGAAAGAGAGGG	AACCGAAAGACCCCTTAACT	55.0	45.0	57.6	56.4	1096-1114 bp	1.0	66.9
trnS-GCU-trnG-UCC_p5	GCTTTAGTCCACTCAGCCAT	AACCGAAAGACCCCTTAACT	50.0	45.0	57.6	56.4	1027-1045 bp	1.0	66.9