Hotspot

ndhA

SSC · 124310-126524 · 2215 bp

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Pi 0.00167

MarkerSeek score 47.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2215
variable_sites: 17
pi: 0.00167
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AACCCAGTCACCATAGGGAA	TGGGAGAAGTTGATCGTTGA	50.0	45.0	57.9	56.5	2337-2344 bp	1.0	68.2
ndhA_p2	AACCCAGTCACCATAGGGAA	TGGGAGAAGTTGATCGTTGAA	50.0	42.9	57.9	57.2	2337-2344 bp	1.0	66.2
ndhA_p3	GAACCCAGTCACCATAGGGA	TGGGAGAAGTTGATCGTTGA	55.0	45.0	58.4	56.5	2338-2345 bp	1.0	65.2
ndhA_p4	AACCCAGTCACCATAGGGAA	ATGGGAGAAGTTGATCGTTGA	50.0	42.9	57.9	57.0	2338-2345 bp	1.0	64.4
ndhA_p5	AACCCAGTCACCATAGGGAA	CACCTGGTTTCATCAATTTGCA	50.0	40.9	57.9	58.0	2433-2440 bp	1.0	64.2