Hotspot

clpP

LSC · 73543-75590 · 2048 bp

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Pi 0.00154

MarkerSeek score 56.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2048
variable_sites: 19
pi: 0.00154
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TTTAGATCGATCCTAACCGG	ACGTTTCCACATCAAAGTGA	45.0	40.0	54.1	55.5	2216-2224 bp	1.0	41.0
clpP_p2	TTTAGATCGATCCTAACCGG	ACGTTTCCACATCAAAGTGAA	45.0	38.1	54.1	56.3	2216-2224 bp	1.0	41.0
clpP_p3	TTTAGATCGATCCTAACCGG	ACGTTTCCACATCAAAGTGAAA	45.0	36.4	54.1	57.0	2216-2224 bp	1.0	41.0
clpP_p4	TTTAGATCGATCCTAACCGG	ACCATTGTTTGTTACGTTTCCA	45.0	36.4	54.1	57.0	2229-2237 bp	1.0	41.0
clpP_p5	TTTAGATCGATCCTAACCGG	TGTTACGTTTCCACATCAAAGTG	45.0	39.1	54.1	57.8	2220-2228 bp	1.0	41.0