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rpl32-trnL-UAG

SSC · 117394-118779 · 1386 bp

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Pi 0.00336

MarkerSeek score 58.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1386
variable_sites: 22
pi: 0.00336
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCTACCGGGCATTCCAAAAG	TTTAGAAGATGCCGCCACTC	50.0	50.0	57.9	58.0	1528-1536 bp	1.0	81.0
rpl32-trnL-UAG_p2	CTACCGGGCATTCCAAAAGT	TTTAGAAGATGCCGCCACTC	50.0	50.0	58.2	58.0	1527-1535 bp	1.0	80.6
rpl32-trnL-UAG_p3	TCTACCGGGCATTCCAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	1478-1486 bp	1.0	80.2
rpl32-trnL-UAG_p4	CTACCGGGCATTCCAAAAGT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	1477-1485 bp	1.0	79.9
rpl32-trnL-UAG_p5	CGCGGCAAAAGCTTTATCTT	TTTAGAAGATGCCGCCACTC	45.0	50.0	57.7	58.0	1562-1570 bp	1.0	79.8