Hotspot

trnF-GAA-ndhJ

LSC · 50372-50925 · 554 bp

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Pi 0.00093

MarkerSeek score 48.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 554
variable_sites: 3
pi: 0.00093
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	710-728 bp	1.0	81.8
trnF-GAA-ndhJ_p2	TCCTCTAATAGGGCGATGCA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.0	58.1	742-760 bp	1.0	81.6
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	674-692 bp	1.0	81.5
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	698-716 bp	1.0	80.7
trnF-GAA-ndhJ_p5	TGACATAGACCCCAGTCCTC	ATCCACGCCTGAAACGTATC	55.0	50.0	57.8	58.1	757-775 bp	1.0	80.4