Hotspot

trnE-UUC-trnT-GGU

LSC · 32948-33671 · 724 bp

Back to Machilus

Pi 0.000969
MarkerSeek score 48.90
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
724
variable_sites
6
pi
0.000969
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CCATGATCAACCCCTTTCGT GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 871-882 bp 1.0 79.8
trnE-UUC-trnT-GGU_p2 CAACCCCTTTCGTCTTACCC GAACCGATGACTTACGCCTT 55.0 50.0 58.2 58.0 864-875 bp 1.0 79.4
trnE-UUC-trnT-GGU_p3 CCATGATCAACCCCTTTCGT CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 850-861 bp 1.0 79.4
trnE-UUC-trnT-GGU_p4 CCATGATCAACCCCTTTCGT TGGCGTTACTCTACCACTGA 50.0 50.0 57.9 58.1 847-858 bp 1.0 79.2
trnE-UUC-trnT-GGU_p5 CAACCCCTTTCGTCTTACCC CCATGGCGTTACTCTACCAC 55.0 55.0 58.2 58.1 843-854 bp 1.0 79.0