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psbM-trnD-GUC

LSC · 31359-32351 · 993 bp

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Pi 0.00119

MarkerSeek score 48.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 993
variable_sites: 6
pi: 0.00119
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAACGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	42.9	50.0	58.2	57.9	1106-1148 bp	1.0	71.5
psbM-trnD-GUC_p2	GAATGAACAACGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	56.7	57.9	1105-1147 bp	1.0	71.4
psbM-trnD-GUC_p3	AGAATGAACAACGCAGTAGCA	ATGCTGTACACCCCATTTCC	42.9	50.0	58.2	57.9	1135-1177 bp	1.0	71.0
psbM-trnD-GUC_p4	GAATGAACAACGCAGTAGCA	ATGCTGTACACCCCATTTCC	45.0	50.0	56.7	57.9	1134-1176 bp	1.0	70.9
psbM-trnD-GUC_p5	GAATGAACAACGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	42.9	50.0	57.5	57.9	1105-1147 bp	1.0	69.1