Hotspot

rps16-trnQ-UUG

LSC · 6303-8174 · 1872 bp

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Pi 0.00118
MarkerSeek score 48.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1872
variable_sites
13
pi
0.00118
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1931-1946 bp 1.0 79.5
rps16-trnQ-UUG_p2 GGGTACAAGAATCCACAGCC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.2 58.1 1964-1979 bp 1.0 78.6
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1935-1950 bp 1.0 78.4
rps16-trnQ-UUG_p4 CCACAGCCGATAATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 1952-1967 bp 1.0 77.9
rps16-trnQ-UUG_p5 GTACAAGAATCCACAGCCGA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 1962-1977 bp 1.0 77.5