Hotspot

rps16

LSC · 5305-6394 · 1090 bp

Back to Macadamia

Pi 0.0055

MarkerSeek score 47.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1090
variable_sites: 6
pi: 0.0055
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AATAAGCAAGCTCGACCCTG	TGATTGCGACGACTTGTTGA	50.0	45.0	58.0	58.1	2042-2048 bp	1.0	79.1
rps16_p2	AATAAGCAAGCTCGACCCTG	TGCGACGACTTGTTGATTGA	50.0	45.0	58.0	58.1	2038-2044 bp	1.0	79.1
rps16_p3	CAATAAGCAAGCTCGACCCT	TGATTGCGACGACTTGTTGA	50.0	45.0	58.0	58.1	2043-2049 bp	1.0	79.1
rps16_p4	CAATAAGCAAGCTCGACCCT	TGCGACGACTTGTTGATTGA	50.0	45.0	58.0	58.1	2039-2045 bp	1.0	79.1
rps16_p5	ACTTGTGTTGGATTGGCACT	TGCGACGACTTGTTGATTGA	45.0	45.0	57.9	58.1	2168-2174 bp	1.0	78.7