Hotspot

ndhF

SSC · 114511-116730 · 2220 bp

Back to Macadamia

Pi 0.00405

MarkerSeek score 44.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 9
pi: 0.00405
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	GAGCTGGCGAATCGGAAATA	50.0	50.0	58.0	58.1	2691 bp	1.0	79.2
ndhF_p2	ATGCCGTCACCTATTGTCAC	GAGCTGGCGAATCGGAAATA	50.0	50.0	58.0	58.1	2796 bp	1.0	79.1
ndhF_p3	ACAGGAACAAGAGGGATCCA	GAGCTGGCGAATCGGAAATA	50.0	50.0	58.0	58.1	2677 bp	1.0	79.0
ndhF_p4	AAGAGGGATCCACCGAAGAA	GAGCTGGCGAATCGGAAATA	50.0	50.0	58.0	58.1	2669 bp	1.0	78.9
ndhF_p5	CGGAAGAAAGGGAGCAAAGT	GAGCTGGCGAATCGGAAATA	50.0	50.0	58.1	58.1	2750 bp	1.0	78.5