Hotspot

ndhF-rpl32

SSC · 116731-117832 · 1102 bp

Back to Macadamia

Pi 0.00457

MarkerSeek score 62.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1102
variable_sites: 5
pi: 0.00457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTCGGAACAAGGAGAAGTCC	AAAAGCTTTTAACGCTGCCC	55.0	45.0	57.9	57.9	1281-1290 bp	1.0	79.0
ndhF-rpl32_p2	CGTCGGAACAAGGAGAAGTC	AAAAGCTTTTAACGCTGCCC	55.0	45.0	58.3	57.9	1282-1291 bp	1.0	77.3
ndhF-rpl32_p3	TGGAAGTGGAAGGAGTGGTA	AAAAGCTTTTAACGCTGCCC	50.0	45.0	57.6	57.9	1240-1249 bp	1.0	76.4
ndhF-rpl32_p4	GTCGGAACAAGGAGAAGTCC	TTTAACGCTGCCCAATACCC	55.0	50.0	57.9	58.5	1274-1283 bp	1.0	75.9
ndhF-rpl32_p5	GTCGGAACAAGGAGAAGTCC	AGCTTTTAACGCTGCCCAAT	55.0	45.0	57.9	58.7	1278-1287 bp	1.0	74.3