Hotspot

trnT-GGU-psbD

LSC · 34910-36540 · 1631 bp

Back to Macadamia

Pi 0.00431

MarkerSeek score 61.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1631
variable_sites: 7
pi: 0.00431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	ATAGTGGCTAGCGGCTGATA	CCGCAACCAGTCATCCATAA	50.0	50.0	58.1	58.0	1812-1816 bp	1.0	79.7
trnT-GGU-psbD_p2	TAGTGGCTAGCGGCTGATAT	CCGCAACCAGTCATCCATAA	50.0	50.0	58.1	58.0	1811-1815 bp	1.0	79.7
trnT-GGU-psbD_p3	GCCCTTTTAACTCAGCGGTA	CCGCAACCAGTCATCCATAA	50.0	50.0	57.9	58.0	1771-1775 bp	1.0	79.5
trnT-GGU-psbD_p4	GCTAGCGGCTGATATTCAGG	CCGCAACCAGTCATCCATAA	55.0	50.0	58.3	58.0	1806-1810 bp	1.0	78.0
trnT-GGU-psbD_p5	GGCTAGCGGCTGATATTCAG	CCGCAACCAGTCATCCATAA	55.0	50.0	58.3	58.0	1807-1811 bp	1.0	78.0