Hotspot

ndhA

SSC · 119839-122143 · 2305 bp

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Pi 0.00304

MarkerSeek score 59.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2305
variable_sites: 7
pi: 0.00304
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAAGGAACA	TCCGCCGGGTTTTATCAATT	42.9	45.0	56.3	57.9	2527-2739 bp	1.0	57.4
ndhA_p2	AACCCAGTTACCATAAGGAACA	TCCGCCGGGTTTTATCAATT	40.9	45.0	57.0	57.9	2528-2740 bp	1.0	55.3
ndhA_p3	TGAACCCAGTTACCATAAGGA	TCCGCCGGGTTTTATCAATT	42.9	45.0	56.0	57.9	2530-2742 bp	1.0	55.1
ndhA_p4	GAACCCAGTTACCATAAGGAACA	TCCGCCGGGTTTTATCAATT	43.5	45.0	58.1	57.9	2529-2741 bp	1.0	54.7
ndhA_p5	TGAACCCAGTTACCATAAGGAAC	TCCGCCGGGTTTTATCAATT	43.5	45.0	58.1	57.9	2530-2742 bp	1.0	54.7