Hotspot

ndhD-psaC

SSC · 117167-117288 · 122 bp

Back to Maackia

Pi 0.0656

MarkerSeek score 83.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 122
variable_sites: 8
pi: 0.0656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GAAGATTAAGGAACCCCCGG	AAACAACTCGAAGCATGGGT	55.0	45.0	57.7	58.0	220 bp	1.0	80.0
ndhD-psaC_p2	GAAGATTAAGGAACCCCCGG	GGCATGAAACAACTCGAAGC	55.0	50.0	57.7	58.0	226 bp	1.0	79.8
ndhD-psaC_p3	GAAGATTAAGGAACCCCCGG	GTGTGAATCTGCCTGTCCAA	55.0	50.0	57.7	58.1	276 bp	1.0	78.7
ndhD-psaC_p4	GAAGATTAAGGAACCCCCGG	TGTGAATCTGCCTGTCCAAC	55.0	50.0	57.7	58.1	275 bp	1.0	78.7
ndhD-psaC_p5	GAAGATTAAGGAACCCCCGG	GAGGTGTGAATCTGCCTGTC	55.0	55.0	57.7	58.3	279 bp	1.0	77.4