Hotspot

petA-psbJ

LSC · 62831-64014 · 1184 bp

Back to Maackia

Pi 0.0127

MarkerSeek score 66.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1184
variable_sites: 15
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	CATATTCCGGATTGGGCTCA	50.0	50.0	57.8	57.4	1324 bp	1.0	74.4
petA-psbJ_p2	CGTTACGTGTCCAAGGTCTC	CATATTCCGGATTGGGCTCA	55.0	50.0	58.3	57.4	1329 bp	1.0	73.9
petA-psbJ_p3	GATCCGTTACGTGTCCAAGG	CATATTCCGGATTGGGCTCA	55.0	50.0	58.4	57.4	1333 bp	1.0	73.5
petA-psbJ_p4	CGTGTCCAAGGTCTCTTGTT	TATTCCGGATTGGGCTCATC	50.0	50.0	57.8	57.1	1322 bp	1.0	72.1
petA-psbJ_p5	CGTGTCCAAGGTCTCTTGTT	TCATATTCCGGATTGGGCTC	50.0	50.0	57.8	57.1	1325 bp	1.0	72.1