Hotspot

trnG-GCC-trnfM-CAU

LSC · 46081-46198 · 118 bp

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Pi 0.0169
MarkerSeek score 62.20
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
118
variable_sites
2
pi
0.0169
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 TCTTTGCCAAGGAGAAGACG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 196 bp 1.0 79.2
trnG-GCC-trnfM-CAU_p2 TCTTTGCCAAGGAGAAGACG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.8 58.0 232 bp 1.0 79.1
trnG-GCC-trnfM-CAU_p3 TCTTTGCCAAGGAGAAGACG GTCACGGGTTCAAATCCTGT 50.0 50.0 57.8 58.1 190 bp 1.0 78.6
trnG-GCC-trnfM-CAU_p4 TCTTTGCCAAGGAGAAGACG TCACGGGTTCAAATCCTGTC 50.0 50.0 57.8 57.8 189 bp 1.0 78.1
trnG-GCC-trnfM-CAU_p5 TCTTTGCCAAGGAGAAGACG TGAGGTCACGGGTTCAAATC 50.0 50.0 57.8 57.8 194 bp 1.0 78.0