Hotspot

trnT-UGU-trnL-UAA

LSC · 34547-35971 · 1425 bp

Back to Maackia

Pi 0.00281

MarkerSeek score 60.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1425
variable_sites: 4
pi: 0.00281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	1483-1490 bp	1.0	78.6
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	1515-1522 bp	1.0	77.7
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	1489-1496 bp	1.0	77.2
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	1521-1528 bp	1.0	76.4
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	TACCAATTTCGCCATACCCC	55.0	50.0	57.9	57.6	1476-1483 bp	1.0	76.3