Hotspot

trnR-UCU-trnG-UCC

LSC · 31509-31692 · 184 bp

Back to Maackia

Pi 0.00578
MarkerSeek score 60.30
Primer available yes
Label trnR-UCU-trnG-UCC

Feature Metadata

label_name
trnR-UCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
184
variable_sites
1
pi
0.00578
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnG-UCC_p1 TGCGCCCAATAGGATTTGAA AGCCTTCCAAGCTAACGATG 45.0 50.0 57.9 58.0 289-300 bp 1.0 81.9
trnR-UCU-trnG-UCC_p2 TTGCGCCCAATAGGATTTGA AGCCTTCCAAGCTAACGATG 45.0 50.0 57.9 58.0 290-301 bp 1.0 81.9
trnR-UCU-trnG-UCC_p3 TGCGCCCAATAGGATTTGAA CCTAGCCTTCCAAGCTAACG 45.0 55.0 57.9 58.1 292-303 bp 1.0 81.7
trnR-UCU-trnG-UCC_p4 TTGCGCCCAATAGGATTTGA CCTAGCCTTCCAAGCTAACG 45.0 55.0 57.9 58.1 293-304 bp 1.0 81.7
trnR-UCU-trnG-UCC_p5 TGCGCCCAATAGGATTTGAA CCCTAGCCTTCCAAGCTAAC 45.0 55.0 57.9 57.7 293-304 bp 1.0 79.5