Hotspot

trnV-UAC-ndhC

LSC · 10265-10678 · 414 bp

Back to Maackia

Pi 0.00483

MarkerSeek score 57.70

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 2
pi: 0.00483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	AGAAGGTCTACGGTTCGAGT	CGGGGCTAAAAGTCCAGAAA	50.0	50.0	57.8	57.8	546 bp	1.0	77.2
trnV-UAC-ndhC_p2	AGAAGGTCTACGGTTCGAGT	CCTTGCTAATCGGGGCTAAA	50.0	50.0	57.8	57.7	556 bp	1.0	76.0
trnV-UAC-ndhC_p3	AGAAGGTCTACGGTTCGAGT	AATCGGGGCTAAAAGTCCAG	50.0	50.0	57.8	57.6	549 bp	1.0	75.4
trnV-UAC-ndhC_p4	GAAGGTCTACGGTTCGAGTC	CGGGGCTAAAAGTCCAGAAA	55.0	50.0	57.5	57.8	545 bp	1.0	74.4
trnV-UAC-ndhC_p5	AGAAGGTCTACGGTTCGAGT	TCGGGGCTAAAAGTCCAGAA	50.0	50.0	57.8	58.6	547 bp	1.0	73.5