Hotspot

ycf1

SSC · 123788-129238 · 5451 bp

Back to Lysionotus

Pi 0.02

MarkerSeek score 56.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5451
variable_sites: 391
pi: 0.02
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCTCTGTTCGTGCGTTCTC	AAATTCGCGCAAGAAAAGGG	50.0	45.0	58.0	57.9	418 bp	0.416667	60.6
ycf1_p2	ATCTCTGTTCGTGCGTTCTC	GTATCCCCCGACCTCTACAA	50.0	55.0	58.0	57.9	1016-1031 bp	0.416667	58.6
ycf1_p3	CGGGGTGAACCAAAAAGAGA	AAATTCGCGCAAGAAAAGGG	50.0	45.0	58.0	57.9	829-835 bp	0.333333	54.0
ycf1_p4	TCGGGGTGAACCAAAAAGAG	AAATTCGCGCAAGAAAAGGG	50.0	45.0	58.0	57.9	830-836 bp	0.333333	54.0
ycf1_p5	CGGGGTGAACCAAAAAGAGA	GTATCCCCCGACCTCTACAA	50.0	55.0	58.0	57.9	1433-1442 bp	0.333333	53.4