Hotspot

ccsA-ndhD

SSC · 115383-115623 · 241 bp

Back to Lysionotus

Pi 0.0354

MarkerSeek score 74.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 241
variable_sites: 30
pi: 0.0354
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CAATTCAGCAATTGTGGCGT	TCAGTTGAAAAGGTTGAAGT	45.0	35.0	57.9	52.8	405-429 bp	1.0	49.7
ccsA-ndhD_p2	CAATTCAGCAATTGTGGCGT	TCAGTTGAAAAGGTTGAAGTT	45.0	33.3	57.9	53.8	405-429 bp	1.0	49.7
ccsA-ndhD_p3	CAATTCAGCAATTGTGGCGT	TCACTATCAGTTGAAAAGGTTGA	45.0	34.8	57.9	55.8	411-435 bp	1.0	49.6
ccsA-ndhD_p4	CAATTCAGCAATTGTGGCGT	CACTATCAGTTGAAAAGGTTGAAGT	45.0	36.0	57.9	57.7	410-434 bp	1.0	49.6
ccsA-ndhD_p5	TTCAGCAATTGTGGCGTCTA	TCAGTTGAAAAGGTTGAAGT	45.0	35.0	57.8	52.8	402-426 bp	1.0	49.6