Hotspot

rpl20-rps12

LSC · 70149-70972 · 824 bp

Back to Lysionotus

Pi 0.00906

MarkerSeek score 57.50

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 824
variable_sites: 31
pi: 0.00906
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	976-982 bp	1.0	77.8
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	975-981 bp	1.0	77.8
rpl20-rps12_p3	TCCGAGCTATATATCCGCGT	AAATGTCACGAAATCCCCCG	50.0	50.0	58.0	58.5	921-927 bp	0.958333	76.8
rpl20-rps12_p4	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	938-944 bp	1.0	76.4
rpl20-rps12_p5	TCCGAGCTATATATCCGCGT	CAGCGTCGAGGAACATGTAC	50.0	55.0	58.0	58.7	884-890 bp	0.958333	75.4