Hotspot

ndhC-trnV-UAC

LSC · 51524-52584 · 1061 bp

Back to Lysionotus

Pi 0.0108

MarkerSeek score 63.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1061
variable_sites: 51
pi: 0.0108
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGCCAAAATGGGAATAAGACTTG	GCACGTTGGGTCTTTGAAAC	39.1	50.0	57.6	58.2	1142-1241 bp	1.0	58.8
ndhC-trnV-UAC_p2	TGCCAAAATGGGAATAAGACTTG	AGAAGGTCTACGGTTCGAGT	39.1	50.0	57.6	57.8	1078-1177 bp	1.0	58.7
ndhC-trnV-UAC_p3	TGCCAAAATGGGAATAAGACTTG	AATGCACGTTGGGTCTTTGA	39.1	45.0	57.6	58.3	1145-1244 bp	1.0	57.6
ndhC-trnV-UAC_p4	TGCCAAAATGGGAATAAGACTTG	ATGCACGTTGGGTCTTTGAA	39.1	45.0	57.6	58.3	1144-1243 bp	1.0	57.6
ndhC-trnV-UAC_p5	TGCCAAAATGGGAATAAGACT	GCACGTTGGGTCTTTGAAAC	38.1	50.0	55.4	58.2	1142-1241 bp	1.0	57.6