Hotspot

trnT-GGU-psbD

LSC · 32301-33636 · 1336 bp

Back to Lysionotus

Pi 0.0113

MarkerSeek score 61.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1336
variable_sites: 67
pi: 0.0113
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1490-1533 bp	1.0	83.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1493-1536 bp	1.0	82.8
trnT-GGU-psbD_p3	GCTCAGTGGTAGAGTAACGC	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1495-1538 bp	1.0	82.8
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1480-1523 bp	1.0	82.0
trnT-GGU-psbD_p5	AAGCCCTTTTAGCTCAGTGG	GGACCAGCCTACAAAAACGA	50.0	50.0	57.8	58.1	1506-1549 bp	1.0	81.9