Hotspot

trnE-UUC-trnT-GGU

LSC · 31533-32228 · 696 bp

Back to Lysionotus

Pi 0.0133

MarkerSeek score 61.60

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 696
variable_sites: 36
pi: 0.0133
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	753-948 bp	1.0	86.2
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	732-927 bp	1.0	85.9
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	729-924 bp	1.0	85.7
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	750-945 bp	1.0	85.1
trnE-UUC-trnT-GGU_p5	TTTCGTAGTACCCTACCCCC	GCGTTACTCTACCACTGAGC	55.0	55.0	57.8	58.1	811 bp	0.041667	39.7