Hotspot

trnG-UCC-trnR-UCU

LSC · 10207-10415 · 209 bp

Back to Lysionotus

Pi 0.0254

MarkerSeek score 57.00

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 209
variable_sites: 19
pi: 0.0254
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	TAACGATGCGGGTTCGATTC	TGTCCTATCCATTAGACAATG	50.0	38.1	58.4	52.4	256-267 bp	1.0	49.8
trnG-UCC-trnR-UCU_p2	CTAACGATGCGGGTTCGATT	TGTCCTATCCATTAGACAATG	50.0	38.1	58.4	52.4	257-268 bp	1.0	49.7
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	TGTCCTATCCATTAGACAATG	50.0	38.1	58.0	52.4	268-279 bp	1.0	49.5
trnG-UCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	TGTCCTATCCATTAGACAATG	55.0	38.1	58.1	52.4	271-282 bp	1.0	49.5
trnG-UCC-trnR-UCU_p5	CCCTAGCCTTCCAAGCTAAC	TGTCCTATCCATTAGACAATG	55.0	38.1	57.7	52.4	272-283 bp	1.0	49.4