Hotspot

rps16-trnQ-UUG

LSC · 6213-7301 · 1089 bp

Back to Lysionotus

Pi 0.0212

MarkerSeek score 63.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1089
variable_sites: 117
pi: 0.0212
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGATGTCGAGACAAGAGC	TGAAGCCACCCAATCACAAA	50.0	45.0	58.0	57.9	1266-1584 bp	1.0	90.2
rps16-trnQ-UUG_p2	AACGATGTCGAGACAAGAGC	TTGAAGCCACCCAATCACAA	50.0	45.0	58.0	57.9	1267-1585 bp	1.0	90.2
rps16-trnQ-UUG_p3	AACGATGTCGAGACAAGAGC	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.0	58.1	1540-1856 bp	1.0	89.6
rps16-trnQ-UUG_p4	AACGATGTCGAGACAAGAGC	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	1545-1861 bp	1.0	89.6
rps16-trnQ-UUG_p5	AACGATGTCGAGACAAGAGC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	975-1293 bp	0.791667	81.3