Hotspot

ycf1

SSC · 123669-129236 · 5568 bp

Back to Lysimachia

Pi 0.0312

MarkerSeek score 70.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5568
variable_sites: 1105
pi: 0.0312
unique_haplotype_count: 61
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGCGTTTTAGTTTCGCTTG	TGGATGAGAAAGGGAGCAGA	45.0	50.0	58.0	58.0	798 bp	0.015873	40.2
ycf1_p2	ACGCGTTTTAGTTTCGCTTG	AAAACAGACGAGGTGGCTTT	45.0	45.0	58.0	57.9	1011 bp	0.015873	40.1
ycf1_p3	ACGCGTTTTAGTTTCGCTTG	GTCTAAAACCTTGGCACGGA	45.0	50.0	58.0	58.1	401 bp	0.015873	39.6
ycf1_p4	ACGCGTTTTAGTTTCGCTTG	GGCACGGATCTAAGCTAAGG	45.0	55.0	58.0	57.8	389 bp	0.015873	39.3
ycf1_p5	ACGCGTTTTAGTTTCGCTTG	CGACTGAGAAAGAAAGGCCA	45.0	50.0	58.0	57.8	354 bp	0.015873	39.2