Hotspot

petA-psbJ

LSC · 62799-63743 · 945 bp

Back to Lysimachia

Pi 0.0272

MarkerSeek score 77.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 945
variable_sites: 212
pi: 0.0272
unique_haplotype_count: 60
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCATCTGTTGTTTTGGCACA	TGGTTCATATTCTGGATTGGGT	45.0	40.9	57.8	57.1	938-1578 bp	1.0	67.2
petA-psbJ_p2	GCATCTGTTGTTTTGGCACA	ATTCTGGATTGGGTTCATCC	45.0	45.0	57.8	55.1	930-1570 bp	1.0	66.6
petA-psbJ_p3	AGCATCTGTTGTTTTGGCAC	TGGTTCATATTCTGGATTGGGT	45.0	40.9	57.5	57.1	939-1579 bp	1.0	64.9
petA-psbJ_p4	GCATCTGTTGTTTTGGCACA	TGGTTCATATTCTGGATTGGGTT	45.0	39.1	57.8	57.8	938-1578 bp	1.0	64.6
petA-psbJ_p5	AGCATCTGTTGTTTTGGCAC	ATTCTGGATTGGGTTCATCC	45.0	45.0	57.5	55.1	931-1571 bp	1.0	64.3