Hotspot

trnT-GGU-psbD

LSC · 31320-32527 · 1208 bp

Back to Lysimachia

Pi 0.0339

MarkerSeek score 79.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1208
variable_sites: 264
pi: 0.0339
unique_haplotype_count: 58
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CCCTCCGTAACCAATCATCC	55.0	55.0	58.1	57.7	1257-1439 bp	1.0	89.4
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	CCCTCCGTAACCAATCATCC	50.0	55.0	58.1	57.7	1260-1442 bp	0.984127	88.6
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	CCCTCCGTAACCAATCATCC	50.0	55.0	58.3	57.7	1234-1416 bp	1.0	87.7
trnT-GGU-psbD_p4	GGCCCTTTTAACTCAGTGGT	CCCTCCGTAACCAATCATCC	50.0	55.0	57.7	57.7	1272-1454 bp	1.0	87.6
trnT-GGU-psbD_p5	TGGTAGAGTAACGCCATGGT	CCCTCCGTAACCAATCATCC	50.0	55.0	58.4	57.7	1256-1438 bp	1.0	86.5