Hotspot

petN-psbM

LSC · 28377-28773 · 397 bp

Back to Lysimachia

Pi 0.0711

MarkerSeek score 77.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 397
variable_sites: 116
pi: 0.0711
unique_haplotype_count: 55
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TCTCGTGCCTACTGCTTTTC	50.0	50.0	58.2	57.9	531-1149 bp	1.0	90.1
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	CTCGTGCCTACTGCTTTTCT	50.0	50.0	58.2	57.9	530-1148 bp	1.0	90.1
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	AAGGGTCGTCCGATGATACT	50.0	50.0	58.2	57.9	917-1538 bp	0.984127	89.6
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	AGGGTCGTCCGATGATACTT	50.0	50.0	58.2	57.9	916-1537 bp	0.984127	89.6
petN-psbM_p5	TTGACTCTGCGCCATTGATT	AGGGTCGTCCGATGATACTT	45.0	50.0	58.2	57.9	1011-1632 bp	0.952381	88.1