Hotspot

trnC-GCA-petN

LSC · 27856-28286 · 431 bp

Back to Lysimachia

Pi 0.0326

MarkerSeek score 68.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 431
variable_sites: 98
pi: 0.0326
unique_haplotype_count: 48
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	417-499 bp	1.0	87.0
trnC-GCA-petN_p2	AGTTCAAATCTGGGTGTCGC	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.5	57.6	478-560 bp	1.0	85.4
trnC-GCA-petN_p3	AGTTCAAATCTGGGTGTCGC	ATTAAACCAGCCCAAGCGAG	50.0	50.0	58.5	58.5	425-507 bp	1.0	83.9
trnC-GCA-petN_p4	AGTTCAAATCTGGGTGTCGC	ACCATTAAACCAGCCCAAGC	50.0	50.0	58.5	58.7	428-510 bp	1.0	82.3
trnC-GCA-petN_p5	TTCAAATCTGGGTGTCGCC	AGCCCAAGCGAGACTTACTA	52.6	50.0	58.1	58.2	415-497 bp	1.0	82.3