Hotspot

rpoB-trnC-GCA

LSC · 26637-27784 · 1148 bp

Back to Lysimachia

Pi 0.0353

MarkerSeek score 77.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1148
variable_sites: 260
pi: 0.0353
unique_haplotype_count: 58
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCTTCATTTCCATCCCGGA	CTTACCACTCGGCCATGTC	50.0	57.9	58.4	57.9	947-1237 bp	0.952381	78.3
rpoB-trnC-GCA_p2	TTCCTTCATTTCCATCCCGG	CTTACCACTCGGCCATGTC	50.0	57.9	57.6	57.9	948-1238 bp	0.952381	78.1
rpoB-trnC-GCA_p3	TCCTTCATTTCCATCCCGGA	AAAGGATTTGCAGTCCCCC	50.0	52.6	58.4	57.6	968-1258 bp	0.952381	75.8
rpoB-trnC-GCA_p4	TTCCTTCATTTCCATCCCGG	AAAGGATTTGCAGTCCCCC	50.0	52.6	57.6	57.6	969-1259 bp	0.952381	75.6
rpoB-trnC-GCA_p5	ATTCCTTCATTTCCATCCCGG	CTTACCACTCGGCCATGTC	47.6	57.9	58.1	57.9	949-1239 bp	0.952381	73.2