Hotspot

ccsA-ndhD

SSC · 119416-119679 · 264 bp

Back to Lysidice

Pi 0.0536

MarkerSeek score 70.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 264
variable_sites: 14
pi: 0.0536
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGTTCATTTACATCAAATTGA	CAGTTGAGAAGGTCGAAGCT	27.3	50.0	52.3	57.6	327-328 bp	1.0	44.2
ccsA-ndhD_p2	TGGTTCATTTACATCAAATTGA	TCAGTTGAGAAGGTCGAAGC	27.3	50.0	52.3	57.6	328-329 bp	1.0	44.1
ccsA-ndhD_p3	ATGGTTCATTTACATCAAATTGA	TCAGTTGAGAAGGTCGAAGC	26.1	50.0	52.9	57.6	329-330 bp	1.0	44.1
ccsA-ndhD_p4	ATGGTTCATTTACATCAAATTGA	CAGTTGAGAAGGTCGAAGCT	26.1	50.0	52.9	57.6	328-329 bp	1.0	44.1
ccsA-ndhD_p5	TGGTTCATTTACATCAAATTGA	ATCAGTTGAGAAGGTCGAAGC	27.3	47.6	52.3	58.0	329-330 bp	1.0	44.1