Hotspot

rps3-rps19

LSC · 86889-87579 · 691 bp

Back to Lysidice

Pi 0.0279

MarkerSeek score 64.40

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 691
variable_sites: 19
pi: 0.0279
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TACCAAGTCTGAAACCGAGC	GGGGACACGCCAAAAATGAT	50.0	50.0	57.5	59.1	769-801 bp	1.0	70.4
rps3-rps19_p2	GTCTGAAACCGAGCGGATTT	GGGGACACGCCAAAAATGAT	50.0	50.0	58.6	59.1	763-795 bp	1.0	69.6
rps3-rps19_p3	TACCAAGTCTGAAACCGAGC	GGGACACGCCAAAAATGATA	50.0	45.0	57.5	56.4	768-800 bp	1.0	66.4
rps3-rps19_p4	TGGTTGTGCAAACCAAAGAG	GGGGACACGCCAAAAATGAT	45.0	50.0	57.0	59.1	807-839 bp	1.0	66.2
rps3-rps19_p5	TACCAAGTCTGAAACCGAGC	GGGGACACGCCAAAAATGA	50.0	52.6	57.5	58.7	769-801 bp	1.0	66.0