Hotspot

ndhC-trnV-UAC

LSC · 53826-54398 · 573 bp

Back to Lysidice

Pi 0.00962

MarkerSeek score 67.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 573
variable_sites: 5
pi: 0.00962
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGCCAAAATGGGAATAACGC	AGAAGGTCTACGGTTCGAGT	45.0	50.0	57.3	57.8	646-678 bp	1.0	77.7
ndhC-trnV-UAC_p2	CTCTGGCCCTTTGCTAATCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.7	57.8	691-723 bp	1.0	77.4
ndhC-trnV-UAC_p3	TCTCTGGCCCTTTGCTAATC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.3	57.8	692-724 bp	1.0	77.2
ndhC-trnV-UAC_p4	GCCAAAATGGGAATAACGCT	AGAAGGTCTACGGTTCGAGT	45.0	50.0	57.0	57.8	645-677 bp	1.0	75.4
ndhC-trnV-UAC_p5	TTTCTCTGGCCCTTTGCTAA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	57.0	57.8	694-726 bp	1.0	75.2