Hotspot

trnR-UCU-atpA

LSC · 10268-10653 · 386 bp

Back to Lysidice

Pi 0.0389

MarkerSeek score 65.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 15
pi: 0.0389
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	AAGCAGAAGCCCTTGTGAAA	47.6	45.0	57.3	57.9	488-516 bp	1.0	68.3
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	CATTTACCGGGGAAGCAGAA	47.6	50.0	57.3	57.9	500-528 bp	1.0	67.8
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AAGACATTTACCGGGGAAGC	47.6	50.0	57.3	57.9	504-532 bp	1.0	67.8
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	AAGCCCTTGTGAAAGAAGCT	47.6	45.0	57.3	57.6	482-510 bp	1.0	65.9
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	AGCAGAAGCCCTTGTGAAAG	47.6	50.0	57.3	58.4	487-515 bp	1.0	65.8