Hotspot

trnR-ACG-ndhB

IRa · 154331-157564 · 3234 bp

Back to Lygodium

Pi 0.0543

MarkerSeek score 78.00

Primer available yes

Label trnR-ACG-ndhB

Feature Metadata

label_name: trnR-ACG-ndhB
feature_type: igs
region: IRa
length_bp: 3234
variable_sites: 449
pi: 0.0543
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-ndhB_p1	CGGGAAGTAAGCAGTCCTTC	GGGAAAGGAACTGCAAGGAA	55.0	50.0	58.0	58.0	716-2618 bp	0.25	58.5
trnR-ACG-ndhB_p2	GGGAAGTAAGCAGTCCTTCG	GGGAAAGGAACTGCAAGGAA	55.0	50.0	58.0	58.0	715-2617 bp	0.25	58.5
trnR-ACG-ndhB_p3	CGGGAAGTAAGCAGTCCTTC	TGACCCAAGAAGCAGCAAAT	55.0	45.0	58.0	58.0	517 bp	0.125	44.9
trnR-ACG-ndhB_p4	GGGAAGTAAGCAGTCCTTCG	TGACCCAAGAAGCAGCAAAT	55.0	45.0	58.0	58.0	516 bp	0.125	44.9
trnR-ACG-ndhB_p5	GGGAAGTAAGCAGTCCTTCG	CCCAAGAAGCAGCAAATTCG	55.0	50.0	58.0	58.0	513 bp	0.125	44.8