Hotspot

rps12-rrn16

IRa · 144766-146796 · 2031 bp

Back to Lygodium

Pi 0.0436
MarkerSeek score 76.60
Primer available yes
Label rps12-rrn16

Feature Metadata

label_name
rps12-rrn16
feature_type
igs
region
IRa
length_bp
2031
variable_sites
306
pi
0.0436
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-rrn16_p1 CTCAGGTCAGCTATGCGTTT CGTCATTGCTTCTTCTCCGA 50.0 50.0 58.0 58.0 2787-2799 bp 0.875 77.8
rps12-rrn16_p2 GAACTCTCGCGACTTTACGT CGTCATTGCTTCTTCTCCGA 50.0 50.0 58.0 58.0 2519-2531 bp 0.875 77.8
rps12-rrn16_p3 CTCAGGTCAGCTATGCGTTT GCCCCACCAACTAGCTAATC 50.0 55.0 58.0 58.0 2587-2599 bp 0.875 77.8
rps12-rrn16_p4 CTCAGGTCAGCTATGCGTTT GGTATTAGCAGCCGTTTCCA 50.0 50.0 58.0 58.0 2514-2526 bp 0.875 77.8
rps12-rrn16_p5 CTCAGGTCAGCTATGCGTTT TAACGCTTGCATCCTCTGTC 50.0 50.0 58.0 58.0 2856-2868 bp 0.875 77.6