Hotspot

ndhG-ndhI

SSC · 120194-120531 · 338 bp

Back to Lygodium

Pi 0.0984

MarkerSeek score 78.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 338
variable_sites: 93
pi: 0.0984
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TACTGTTCTACCGGTGAGCA	CTGCGTTGAATACTGTCCGA	50.0	50.0	58.1	58.0	785-822 bp	0.875	86.2
ndhG-ndhI_p2	TACTGTTCTACCGGTGAGCA	ATGACCAAGTGGCTCTAGGT	50.0	50.0	58.1	58.0	702-739 bp	0.875	86.0
ndhG-ndhI_p3	TACTGTTCTACCGGTGAGCA	TGAGCGTTTTCGTGGAAGAA	50.0	45.0	58.1	58.1	962-999 bp	0.875	85.7
ndhG-ndhI_p4	TACTGTTCTACCGGTGAGCA	GGTAAGTTTTGCGGCATTCC	50.0	50.0	58.1	58.0	1192-1227 bp	0.625	74.7
ndhG-ndhI_p5	TACTGTTCTACCGGTGAGCA	GTAAGTTTTGCGGCATTCCC	50.0	50.0	58.1	58.0	1191-1226 bp	0.625	74.7