Hotspot

rpl32-trnP-GGG

SSC · 114552-115195 · 644 bp

Back to Lygodium

Pi 0.0864

MarkerSeek score 84.80

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: SSC
length_bp: 644
variable_sites: 168
pi: 0.0864
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	TCTTGTCAATCGTGCCCAAA	TGCATCGTGGCGATAAAGAA	45.0	45.0	58.0	58.0	1461 bp	0.125	44.7
rpl32-trnP-GGG_p2	TCTTGTCAATCGTGCCCAAA	TTGCATCGTGGCGATAAAGA	45.0	45.0	58.0	58.0	1462 bp	0.125	44.7
rpl32-trnP-GGG_p3	AGCACAAAAAGACTGGTCGT	TGCATCGTGGCGATAAAGAA	45.0	45.0	58.0	58.0	1663 bp	0.125	44.6
rpl32-trnP-GGG_p4	AGCACAAAAAGACTGGTCGT	TTGCATCGTGGCGATAAAGA	45.0	45.0	58.0	58.0	1664 bp	0.125	44.6
rpl32-trnP-GGG_p5	TCTTGTCAATCGTGCCCAAA	CGTACCATCTTGGGGTGATG	45.0	55.0	58.0	58.0	990 bp	0.125	44.4