Hotspot

rrn16-rps12

IRb · 97600-99630 · 2031 bp

Back to Lygodium

Pi 0.0428

MarkerSeek score 81.60

Primer available yes

Label rrn16-rps12

Feature Metadata

label_name: rrn16-rps12
feature_type: igs
region: IRb
length_bp: 2031
variable_sites: 304
pi: 0.0428
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-rps12_p1	CGTCATTGCTTCTTCTCCGA	GAACTCTCGCGACTTTACGT	50.0	50.0	58.0	58.0	2519-2531 bp	1.0	91.4
rrn16-rps12_p2	GGTATTAGCAGCCGTTTCCA	CTCAGGTCAGCTATGCGTTT	50.0	50.0	58.0	58.0	2514-2526 bp	1.0	91.3
rrn16-rps12_p3	CGTCATTGCTTCTTCTCCGA	CTCAGGTCAGCTATGCGTTT	50.0	50.0	58.0	58.0	2787-2799 bp	1.0	91.2
rrn16-rps12_p4	GCCCCACCAACTAGCTAATC	CTCAGGTCAGCTATGCGTTT	55.0	50.0	58.0	58.0	2587-2599 bp	1.0	91.2
rrn16-rps12_p5	TAACGCTTGCATCCTCTGTC	CTCAGGTCAGCTATGCGTTT	50.0	50.0	58.0	58.0	2856-2868 bp	1.0	91.1