Hotspot

trnI-CAU-trnR-ACG

IRb · 85709-90065 · 4357 bp

Back to Lygodium

Pi 0.0454

MarkerSeek score 78.70

Primer available yes

Label trnI-CAU-trnR-ACG

Feature Metadata

label_name: trnI-CAU-trnR-ACG
feature_type: igs
region: IRb
length_bp: 4357
variable_sites: 517
pi: 0.0454
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-trnR-ACG_p1	GGGAAAGGAACTGCAAGGAA	CGGGAAGTAAGCAGTCCTTC	50.0	55.0	58.0	58.0	716-2618 bp	0.25	58.4
trnI-CAU-trnR-ACG_p2	GGGAAAGGAACTGCAAGGAA	GGGAAGTAAGCAGTCCTTCG	50.0	55.0	58.0	58.0	715-2617 bp	0.25	58.4
trnI-CAU-trnR-ACG_p3	TGACCCAAGAAGCAGCAAAT	CGGGAAGTAAGCAGTCCTTC	45.0	55.0	58.0	58.0	517 bp	0.125	44.9
trnI-CAU-trnR-ACG_p4	TGACCCAAGAAGCAGCAAAT	GGGAAGTAAGCAGTCCTTCG	45.0	55.0	58.0	58.0	516 bp	0.125	44.9
trnI-CAU-trnR-ACG_p5	CCCAAGAAGCAGCAAATTCG	GGGAAGTAAGCAGTCCTTCG	50.0	55.0	58.0	58.0	513 bp	0.125	44.8